Black Bacillus subtilis is a black variant of Bacillus subtilis. Simply put, Bacillus subtilis can produce melanin or black Bacillus subtilis, and there is little difference in stress resistance between the two. Bacillus subtilis is a species of the genus Bacillus. A single cell is 0.7 to 0.8 x 2 to 3 microns and is uniformly colored. No capsule, peripheral flagella, can exercise. Gram-positive bacteria, spores 0.6 ~ 0.9 × 1.0 ~ 1.5 microns, elliptical to columnar, located in the center of the bacteria or slightly biased, the cells do not expand after the formation of spores.
Black Bacillus subtilis commonly used medium formula :
1L distilled water + 20g glucose + 15g peptone + 5g sodium chloride + 0.5g beef extract + 20g agar
Aseptic verification Why do you use black Bacillus subtilis? Because the endospore of Bacillus subtilis is very resistant, various adverse environments can be tolerated, and it is easier to cultivate. Therefore, European and American countries regard it as the test standard, and China is also following international practice. In addition, the black variant of Bacillus subtilis was proposed in 1989 to be divided into a new species. In 2001, it was recognized and accepted by the academic community and divided into one, named "Bacillus licheniformis. Bacillus atrophaeus".
So let's take a look at the characteristics of B. subtilis :
The black variant of Bacillus subtilis belongs to the genus Bacillus, and the vegetative cells are rod-shaped, arranged in a single or short chain, with a cell width of 0.5-1.0 μm and a length of 2.0-4.0 μm. Gram-positive; spore oval, medium-sized, non-expanding On the pancreatic soy agar medium, the colony surface is smooth, opaque, and round. The most prominent sign of this strain is that the colony is brown or brownish red on the medium containing nitrogen.
The Bacillus subtilis black variety (ATCC9372) in this flora is an important strain of Bacillus, which is widely used in agriculture, industry, scientific research, medical and health fields, the United States, Britain, Japan, the European Union and other countries. The strain has been included in the food and medical testing standards as a quality control standard test strain in the region, and the strain has been used as a test indicator for the bactericidal effect evaluation of chemical disinfectants, dry heat, and ethylene oxide.
Since the resistance of B. subtilis is so strong, is there a highly effective fungicide that can kill it? Novo brand sporicide is a fungicide that can effectively kill black Bacillus subtilis, and its kill rate can reach 6 logarithmic values. It can be said that it is a deadly nemesis of B. subtilis .
The Nofos sporicide is the most suitable product for GMP aseptic workshop disinfection. The emergence of sporicide has successfully eliminated the irritating and harmful traditional disinfection concept and broke the inherent concept that air fumigation needs to be left for a period of time.
Mechanism of action:
Hydrogen peroxide is a strong oxidant, and the released oxygen molecules (primary oxygen) oxidize the enzyme system of the microorganism. Glial silver ions impede the basic metabolic function of the bacteria (enzyme) or affect his membrane structure (microactivity). Both of these components target the same goal and work together to improve their performance (potential collaboration). Whether it is hydrogen peroxide or colloidal silver ions, the destruction of all components in the pathogen cells is irreversible, which guarantees the thoroughness of sterilization and, more importantly, the pathogens ensured by this unique mechanism. No resistance will develop.
〠Features】
1. It can meet all disinfection requirements in GMP aseptic area, and effectively kill bacteria, mold, fungi, spores, viruses and various microorganisms.
2, excellent environmental protection, no residue, can be verified: colorless, odorless, no harm to the human body, no irritating odor, no corrosion on the surface, 10 minutes after use of the fumigation process, personnel can enter
3, to achieve sterilization level, verification qualification is complete, kill spores within 3-5 minutes
4, no need to rinse, naturally dried
5, the surface of the sporicide can be used for disinfection and sterilization of the surface of the object
6, aseptic packaging type (ready-to-use type) can be directly brought into the A/B area
7, will not produce resistance
Norfolk has a strong black Bacillus subtilis killing ability, which is effective in killing pathogenic bacteria, spores, fungi and viruses. The effect of Norfolk is nearly 200 kinds of bacteriology, biology, virology and toxicology. Tested and verified (a copy of these test reports is available upon request by the customer).
Novo brand sporicide can kill microorganisms efficiently. It is the deadly nemesis of B. subtilis, so it is very suitable for space sterilization in the clean area or disinfection of the surface of the object. It is the easiest to pass.
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