Pharmaceutical toxicology
1. Toxicity: Intraperitoneal injection of LD20 was 5.5±0.36g/kg in the decoction decoction mice; LD50 was 1.0g/kg and 7.07g/kg in mice injected intraperitoneally with Daphne vinegar and vinegar. The LD50 of its flooding agent was 8.30 kg/kg and 17.78 kg/kg, respectively. The LD50 of intraperitoneal injection of squid decoction in rats was 9.25 g/kg. The LD50 of mice fed with vinegar or phenyl scopolamine was 8.48±1.18g/kg and 14.05±2.03g/kg, respectively. The LD50 of the mice injected intraperitoneally with silk flower and vinegar were 1.0 g/kg and 7.07 g/kg, respectively, while the LD50 of the water infusion was 8.30 g/kg and 17.78 g/kg respectively. Vinegar can reduce the toxicity of oyster blossoms. The LD50 of intraperitoneal injections of ellabanone emulsion and alcohol was 1.8 and 1.9 mg/kg, respectively. The scutellareinol was given to pregnant monkeys daily by intraperitoneal injection of 20-100μg/kg, for 10 consecutive days, the main organs showed obvious lesions, due to diffuse and intravascular coagulation death. Intrauterine injections, subcutaneous injections, or intravenous injections of anthraquinones can cause fever in animals, possibly due to severe chemical inflammation caused by blepharospasm in the placenta, or due to intense contractions during abortion that squeeze amniotic fluid into maternal blood circulation. Caused. Adrenocorticotropic hormone has obvious preventive and therapeutic effects on fever. Aspirin also prevents fever reactions. Sub-acute toxicity test results in rabbits showed that no significant toxicity was observed after continuous gavage for 28 days with vinegar or benzene suspensions of 0.25 and 0.5 g/kg, and 0.2-0.8 mg of Flos Huarani Cream was not administered to the monkey amniotic cavity. Obvious effect. Animal experiments have shown that the combination of Flos sophorae and Glycyrrhiza uralensis, its diuretic and purgative effects are inhibited, and the toxicity is enhanced, suggesting that the two drugs should not be combined. However, another study showed that wolfberry flowers alone or in combination with licorice did not show toxic reactions and deaths to small poisons. Another study reported on the toxicity and mutagenicity of the blending of squid and its counterpart drugs.
2. Effect of concocting on toxicity: Fifty healthy female mice (18-21g) were randomly divided into 5 groups, 10 in each group, and injected with an agent in the abdominal cavity to observe the 72-hour mortality. It can be seen that the LD50 vinegar product is twice as high as the raw product, indicating that the vinegar vinegar pot has played a role in reducing toxicity.
3. Contrary to the influence of drug compatibility on toxicity: Yang Zhili reported that 568 healthy mice (weighing 28-36g) and 301 healthy rabbits (weight 1.5-2.5kg) were selected. All were observed for 7 days before the experiment. The average body temperature was 38.94±0.46-39.73±0.22°C. The coccidial oocysts were used to examine fecal occult blood. The spirit, dietary desire and feces were all normal. The experimental mice and rabbits were matched with males and females. After pre-experiment, they were randomly divided into single-taste group, compatibility group and control group (see Table 10). The experimental results showed that mice were given orally. Flower water immersion decoction (soaked and concentrated after immersed for 24 hours, equivalent to 1g of crude drug per 1ml, the same below) and decoction (filtered and concentrated after boiled twice without boiling, equivalent to 2g of crude drug per 1ml). Continuous observation of the spirit, desire for food, and faeces for 72 hours were unclear. The mice were treated with gavage of licorice and glycyrrhiza with the same amount of decoction. No response was observed after continuous observation for 72 hours. The rabbits were treated with water-soaked decoction and observed at 5, 24, and 28 hours respectively. Body temperature, spirit, and desire for food were observed. Feces are normal. There was no significant change from the healthy control group. The mice were intraperitoneally injected with decoction decoction, decoction decoction, and licorice equivalent decoction. The compatibility group was more toxic than the single-taste group.
4. Mutagenesis test: selection of C, L, T, and G pills using drugs such as peony, kansui, medlar, and licorice, application of a Salmonella typhimurium plus mammalian microsomal enzyme assay system, and recombinant recombination of R. The test was performed on the pellet for mutagenicity testing. The results were obtained without addition of the S9 activation system (made up of polychlorinated biphenyl-induced SD rat liver homogenate S9 plus an auxiliary liquid, and an S9 mixture consisting of 1 ml of S9 per 9 ml of auxiliary liquid. Each 100ml auxiliary solution contains NADP 300mg, G-6-Pl06mg, phosphate buffer 50ml, distilled water 48ml, salt solution 2ml), C, L, T, G pills can not induce TA93 and TA98 strains (histidine-negative typhoid fever) The back mutation of the Salmonella mutant strain (his-s) has reason to believe that the liquid medicine does not exhibit mutagenic activity on the Ames method TA93 and TA900 strains, and that the test compound has a low concentration of Bacillus subtilis regardless of whether or not the S9 activation system is added. The repair function of H17 and the recombination-defective derivative strain M45 had no bacteriostatic effect, and there was weak inhibition at high concentrations, but the effects were equal on M45 and H17. It can be considered that the C, L, T, and G pills had no DNA damage effect.
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