Ruisai small class:
What is the MOI value? How to explore the MOI value?
MOI (Multiplicity of Infection) refers to the proportion of cells infected with a virus. Choosing the appropriate MOI value, the higher the infection efficiency of the virus and the expression level of the target protein, the less the toxicity of the relative cells. For actively dividing cells, such as 293 cells with MOI = 1, more than 95% of the cells can express the gene of interest. For non-dividing cells, such as primary cells, the infection efficiency is low, and the MOI value may reach 200-300. We suggest that by comparing the results of different MOI (such as 0, 1, 5, 10, 50, 100, etc.) infected cells, select the appropriate MOI for experiments: the cultured cells can be inoculated into a 96-well plate, and then the report is carried. The negative control virus of the gene EGFP infects the cells according to the ratio of different MOI values, and the MOI value is explored.
Can I perform lentiviral packaging on the mature sequence of miRNA?
Yes, please provide the sequence information of the mature sequence of the miRNA. We will carry out the construction and packaging of miRNA overexpressing lentiviral vector, titer detection and qPCR detection of miRNA. The details of miRNA virus packaging service can be seen.
What security issues should be paid attention to when using a packaged virus?
The use of the virus is required in a biosafety level 2 (BL2) laboratory where the operator wears overalls, masks and disposable latex gloves. Class II biosafety cabinets should be used in the designated area during operation; the action should be as gentle as possible to avoid aerosols and splashes; the virus-containing waste liquid and utensils can be autoclaved at 121 °C for 30 minutes; use sharps (such as syringes) , blade) must be careful.
How to store and use customized adenovirus products after receiving them?
We use dry ice express delivery to deliver the virus to our customers. After receiving the customer, you should pay attention to the following points:
1) Confirm that the dry ice in the foam box remains, and the ice box keeps the temperature low; 2) Confirm that the contents of the foam box are consistent with the shipping sample list, the adenovirus product is packaged intact, no broken or leaked; 3) the company provides The assembled adenovirus product should be stored in a -70 ° C ultra-low temperature freezer if it is not used for a short time. Used within half a year. 4) The adenovirus product frozen in the -80 ° C ultra-low temperature freezer is taken out before use, melted on ice, and used as soon as possible. If the virus after thawing is temporarily used, the titer is high enough (for example, 10^11-12 IU/ml), and it can be stored for 1~2 weeks at 4 °C.
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