The Age of Absolute Quantification - Leading the New Standards of the Lentivirus Industry
How is the lentivirus on the tall one made (1)
What unit is the lentiviral activity titer used?
Look at TU! Look at TU! Look at TU!
TU = transducing units, which is the number of biologically active virus particles per ml. Other VG/mL, VP/mL are all the number of virus particles, the dead life is counted, a false prosperity, please play free!
How to calibrate the lentiviral activity titer?
Absolute quantification! Absolute quantification! Absolute quantification!
Absolutely quantitative method, it is not allowed! Besides, this "absolute quantification" is a recognized titer detection method for medical grade virus products, Novartis, Juno, these CarT big brothers are used! Not allowed? Can the FDA recognize it if it is not allowed?
Complex and expensive methods, for the sake of accuracy, let us endure!
1. Standard test to draw a standard curve to accurately quantify viral genomic DNA copy number
2. Determination of the 5'LTR-3' LTR viral genome copy number inserted into the genome of the cell
3. Tool cell 293T genome virus signature single copy gene A and host feature single copy gene B
4.TU=N*C/V=(initial cell/infected virus volume)×(2×A gene copy/B gene copy), calculate infection efficiency
5. Do not reverse transcription, do not be affected by RNA degradation or reverse transcription efficiency.
6. If the virus is fluorescent or resistant, then combined with the phenotype
quasi! quasi! quasi! Repeatability levers!
Virgo test dog can not resist the titer calibration method!
Is the lentiviral titer so accurate and useful?
I! @#Â¥%&*......
Someone asked me, why do my cells die with a slow virus?
Why do my black cells appear to be infected with a lentivirus?
Most likely, it is infected! Over infected! Over infected!
Is there a pre-experiment to determine the MOI of the cells? Is there an accurate calculation of virus usage based on virus titer?
The higher the concentration of high-purity virus, the more accurately it is necessary to calculate the titer to avoid insufficient infection or excessive infection, to ensure the best cell state, to continue the downstream experiment, so that it saves time and effort to save the virus! ! !
Finally, the pre-test steps of the infection are detailed, please call me Lei Feng!
Jikai gene leads the new standard of lentivirus industry - the calibration of lentiviral activity has entered a new era, not a quantitative lentivirus, bye! 
Long press and pay attention
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