How to choose the right MBP tag antibody?

E. coli maltose binding protein (MBP) is derived from the protein encoded by the E. coli malE gene and is a member of the bacterial maltose transport system, which is responsible for the uptake and catabolism of maltose. The malE gene product can be fused with various proteins and is an effective medium for expression research. The prokaryotic expression vector of MBP fusion protein has the advantages of high expression efficiency and easy purification. MBP is often widely used as a fusion protein in modern molecular cloning.

How to choose the right MBP tag antibody?

When choosing MBP antibodies, we mainly need according to our own needs, especially for experimental applications. In addition, the more types of applications after an antibody is detected, the greater the choice in use; while the monoclonal antibodies from mouse sources generally have better specificity, stability, and potency than polyclonal antibodies. In addition, the titer of the antibody is also an important aspect to examine the cost performance of the antibody, which is equivalent to the dilution ratio in practical use. A high titer of 100 μl MBP antibody (if the dilution ratio of WB assay is 1:5000, the final use solution is equivalent to 500 ml), 1 ml of MBP antibody with lower potency (if the dilution ratio of WB test is 1:200) The final use solution is equivalent to 200ml).

MBP Antibody Recommendations - MBP Monoclonal Antibody to WB
Product name and description Item number product manual
Anti-MBP Tag Mouse Monoclonal Antibody ( 9Y5) A02070 Details

Why recommend the Abbkine MBP monoclonal antibody (clone 9Y5)?

At present, the MBP antibodies available on the market are domestically packaged or produced. The domestically packaged MBP antibodies are insufficient in quality stability, and after being packaged, their potency is also lost. Some MBP antibodies originating in domestic laboratories have better detection activity, but there are gaps in application types and it is difficult to control the stability between batches, and only a small amount of domestically produced antibodies are also available in China. Polyclonal antibody. There are many imported brands that supply MBP antibodies, such as Abcam, Bio-Rad, Novus, Thermo, etc., which are related to MBP antibodies, but they are far less expensive than Abbkine products.

Brand App types Specifications / price
Abbkine WB: 1:1000-1:10000 ¥605/50ul; ¥1800/200ul; ¥7200/1ml
Abcam WB: 1:5000 ¥4304/100ul
Thermo WB: 1:1000-1:2000 ¥3649/50ul

Therefore, we recommend Abbkine's Anti-MBP Tag Mouse Monoclonal Antibody (9Y5). Like Abcam, Abbkine's MBP antibody immunogen is KLH-conjugated MBP full-length protein. After continuous screening and optimization, we finally get the most. A good cell line (9Y5) recognizes MBP protein and MBP fusion protein overexpressed in cells. As a production brand in the United States, Abbkine provides OEM services to a number of internationally renowned brands, so its price is low and its quality has undergone market testing. This antibody can be used in Western Bloting.

Anti-MBP Tag Mouse Monoclonal Antibody ( 9Y5) Results

In addition to a wide range of applications, Abbkine also offers 1ml large packaging and larger custom-made MBP-labeled antibodies, making it ideal for routine laboratory testing of MBP immunology. For the convenience of customers, Abbkine is available in stock in China. Abbkine provides global product quality tracking and after-sales service and protection for the Chinese market. If you have any problems during the use, please contact your local agent Ai Meijie Technology Co., Ltd. directly.

The American Abbkine brand is a brand that provides cost-effective immunology and cytology research products and solutions. Amy Jie introduced it to China as its regional agent in China, and will provide customers with the most cost-effective routine immunological reagents, such as A tag antibody, an internal reference antibody, a conventional primary antibody, and a full range of secondary antibodies, antibody detection substrates, and related kits. At the same time, as a Chinese agent or regional agent of many international famous brands, Aimei can provide you with a series of laboratory solutions. If you are interested in the above products, please call the free customer line 400-6800-868 to contact Aimei Technology Co., Ltd. for product information and complete experimental solutions.

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