Why choose histone H3 as an internal reference for the nucleus ?
When the experimental sample is only a nuclear protein, not a total cellular extract, a histone H (Histone H), or a proliferating cell nuclear antigen (PCNA) may be used as a nuclear reference antibody. In addition to these, other common nuclear protein internal references are K70, K80, Lamin A and B. However, the problem to be noted is that the choice of nuclear protein internal reference needs to consider the actual experimental environment. For example, in the experiments involving cell proliferation, c-Jun is not suitable for internal reference because of its own expression changes; in the apoptosis experiment, TBP, Lamin Etc. is also not suitable as an internal reference. Conversely, histone Histone H3 is a constitutive component of chromosomes and its expression is relatively stable and is often used as the most extensive nuclear internal reference.
| Product name and description | Item number | product manual | |
| Anti-Histone H3 Mouse Monoclonal Antibody (2D10) | A01070 | Details | |
Why recommend the Abbkine brand histone H3 internal reference antibody (clone 2D10)?
The Histone H3 antibody currently available on the market has been domestically packaged or produced. The domestically packaged Histone H3 antibody has insufficient quality stability, and its valence is also lost after being packaged. Some Histone H3 antibodies native to domestic laboratories have better detection activity and are more difficult to control in batch stability, while only a small number of native antibodies in China are polyclonal antibodies. As for imported brands that supply Histone H3 antibodies, there are not many imported brands. Only Abcam, Sigma and other brands can provide Histone H3 antibodies, but they are far less expensive than Abbkine products.
"Histone H3 Internal Reference Antibody Comprehensive Comparison"
| Brand | Item number | Reactive species | App types | Specifications / price |
| Abbkine | A01070 (mAb) | Human, mouse, rat, yeast | WB: (1:2000-1:5000) IF: (1:100-1:500) IP: (1:200) | 605/50ul 1800/200ul 6020/1ml |
| Abcam | Ab18521 (multi-anti) | Cow, human, yeast, Xenopus laevis | WB: (1:1000) IHC: (1:1000) IP: (1:200) | 4450/100ul |
| Sigma | H9289-200UL (multi-anti) | people | WB: (1:2000-1:5000) | 3945/200ul |
Therefore, we recommend Abbkine's Anti-Histone H3 Mouse Monoclonal Antibody (2D10) . Like Abcam, Abbkine's Histone H3 antibody immunogen is a recombinant Histone H3 full-length protein, which has been continuously screened and optimized to finally get the most A good cell line (2D10), suitable for most mammalian Histone H3 proteins. As a production brand in the United States, Abbkine provides OEM services to a number of internationally renowned brands, so its price is low and its quality has undergone market testing. This antibody can be used for IF, IP, WB .
Antibody verification result graph
(1:2000 dilution ratio; A, HepG2; B, 293T; C, Mouse brain tissue; D, rat brain tissue)
Abbkine also offers a 1ml large package and a larger custom-made Histone H3 antibody, making it ideal for the ongoing testing of Histone H3 immunoassays. For the convenience of customers, Abbkine is available in stock in China. Abbkine provides global product quality tracking and after-sales service and protection for the Chinese market. If you have any problems during use, please contact Abbkine local agent or Aimei Technology Co., Ltd. directly.
| Product name and description | Item number | product manual | |
| Anti-Histone H3 Mouse Monoclonal Antibody (2D10) | A01070 | Details | |
The American Abbkine brand is a brand that provides cost-effective immunology and cytology research products and solutions. Amy Jie introduced it to China as its regional agent in China, and will provide customers with the most cost-effective routine immunological reagents, such as A tag antibody, an internal reference antibody, a conventional primary antibody, and a full range of secondary antibodies, antibody detection substrates, and related kits. At the same time, as a Chinese agent or regional agent of many international famous brands, Aimei can provide you with a series of laboratory solutions. If you are interested in the above products, please call the free customer line 400-6800-868 to contact Aimei Technology Co., Ltd. for product information and complete experimental solutions.
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