The basic working principle of the new generation of Namocell single cell separation technology

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Technical introduction:

The technology uses a new generation of cell separation technology integrating current collecting cytometry and microfluidic technology. The fluid focusing technology of the flow cytometer can greatly improve the detection sensitivity. In addition, the microfluidic technology can reduce the sheath fluid pressure inside the device to below 2 psi without high frequency oscillation, which greatly reduces the damage of the cells during the sorting process. , greatly improving the activity of the sorted cells. In addition, the disposable chip is the first to achieve complete isolation between the samples during the cell sorting process (from sample loading to separation all in the chip).

working principle:

In the microfluidic channel after laser irradiation, an electronic switch is provided to capture the liquid in which the cells of interest are located and to separate them individually.

Sample application scope:

Samples of suspended particles of all sizes below 40 microns, including: cells, yeast, bacteria, phage, etc.

Technical features:

Gentle sorting: 2 psi of sheath fluid pressure to increase sorting cell viability

Flexible sorting: sample concentration range 10 ~ 10 8 cell / ml; can choose very rare samples of one part per million.

Sterile sorting: one-time chip to ensure complete isolation between samples; the instrument is small in size and can be placed in a clean bench

Efficient sorting: 96-well plate sorting is less than 1 min, 384-well plate is less than 4 min, and the monoclonal rate is over 95%.

Easy sorting: the instrument is easy to operate and requires no personnel to operate and maintain

Lightweight sorting: small size, can be placed in a clean platform

Application areas:

Antibody drug development:

- Monoclonal antibody screening

- High-yield cell line selection

- High efficiency 96-well plate single cell sorting

Single cell sequencing:

- Single cell separation

- Single cell CTCs (circulating tumor cells) capture

- Free fetal cell capture

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