1. Effects on phagocytosis of macrophages: mice weighing 18-22 g were administered intraperitoneally with 100 mg/kg sodium alginate solution in the administration group, and the control was treated with the same amount of physiological saline for 7 consecutive days. As a result, sodium alginate was significantly enhanced. The phagocytosis of rat peritoneal macrophages was 1.43 times that of the control group, and the phagocytosis index was 2.96 times that of the control group.
2. The effect on lymphocyte transformation was as follows: normal human heparin anticoagulation (0.2 ml) was added to a test tube containing 2 ml of culture medium and divided into four groups: control, PHA, sodium alginate, and sodium alginate plus PHA. Add 3H-TdR or 3H-UR to each tube, count and calculate the stimulation index SI value. The results showed that compared with the control group, sodium alginate could promote 3H-TdR and 3H-UR infiltrating lymphocytes, but they were all weaker than PHA. In the TdR experiment, sodium alginate and PHA had antagonistic effects; in the 3H-UR experiment , Sodium alginate combined with PHA has an additive effect. The Sl values ​​are 1.000, 3.827, 1.514, 1.840, respectively.
3. The effect on the number of white blood cells: 30 Kunming mice weighing 18-22 g were divided into normal saline, cyclophosphamide (Cy) and drug plus Cy group. The administration group was intraperitoneally injected with 100 mg/kg sodium alginate for 8 days. On the 7th day, except for the normal saline group, each mouse was intraperitoneally injected with 2 mg/0.4 ml of Cy, and the number of white blood cells in each rat was examined on the 9th day. The results showed that sodium alginate had an antagonistic effect on cyclophosphamide-induced neutropenia, with an antagonistic rate of 54.35% and P<0.001 compared with the control group.
4. Effect on hemolysin production in mice: 20 mice weighing 18-22 g were divided into 2 groups. In the administration group, 100 mg/kg of sodium alginate was intraperitoneally injected, and the control was administered with the same amount of physiological saline. Continuous administration for 8 days. The half hemolytic values ​​(HC50) of the control group and the administration group were 7.913±1.335 and 29.29±4.236, respectively (P<0.001), and the HC50 was 3.70 times that of the control group, indicating that sodium alginate has the function of enhancing humoral immunity.
5. The effect of 60Co ray radiation: 40 Kunming mice weighing 18-22g were divided into 2 groups. In the administration group, 100 mg/kg of sodium alginate was intraperitoneally injected for 7 days, and 1 hour after the 8th day of administration, 60 Co ray irradiation was performed for a total of 0.2064 c/kg for 23 minutes and observed for 30 days. RESULTS: Sodium alginate had a protective effect on the damage caused by 60Co-ray irradiation, and it could reduce the mortality and prolong the survival time (P<0.05).
6. The effect of lowering serum cholesterol: 20 mice weighing 20-22 g were divided into 2 groups. The administration group was intraperitoneally injected with 100 mg/kg sodium alginate for 7 days. On the 6th day, 75% egg yolk emulsion 0.5 ml was injected intraperitoneally. Blood was taken 20 hours later and the serum cholesterol content was measured. Results The cholesterol content of the control group was 49.11±56.23 mg/100 ml, and that of the administration group was 311.21±30.87 mg/100 ml, with a decrease rate of 36.50% (P<0.001). Shows that sodium alginate has a significant reduction in serum cholesterol.
7. Anti-tumor effect: S180 was inoculated into 18-22 g mice according to the literature method, and the next day was given by intraperitoneal injection of 200 mg/kg sodium alginate. The control group was treated with the same amount of physiological saline for 9 consecutive days. The animals were sacrificed. The average tumor weight of the control group was 1.807±0.117 g, and that of the control group was 1.151±0.140 g. The inhibition rate was 36.30% (P<0.001). (SFPPR is precipitated with polysaccharide A aqueous solution plus CPC precipitated salt, add ethanol precipitate) and polysaccharide C (SFPPRR, is precipitated with polysaccharide B aqueous solution plus calcium chloride, salt, ethanol precipitate) anti-tumor test results, The tumor inhibition rates of SFPPR in mice S180 and EAC were 48.8% and 38.5%, respectively; the inhibitory rates of SFPPRR were 28.8% and 12%, respectively.
8. Anti-endotoxin effects: polysaccharide A extracted from seaweed (SFPOP, crude polysaccharide aqueous solution plus ethanol isocyanate), polysaccharide B (SFPPR) and polysaccharide c (SFPPRR) against endotoxin poisoning in mice (dose 30 mg/mouse) The survival rates of SFPP, SFPPR, SFPPRR, and control groups were 6/10, 9/10, 3/10, and 0/10, respectively.
9. Effect on Herpes Simplex Virus Type I: The minimum effective dose of algal polysaccharides for type I herpes simplex virus in the simultaneous treatment and extracorporeal administration route is <25 mg/ml (inhibition of the virus logarithm of 2.45), <50 μg/ml ( 2.50), <100 μg/ml (2.25). Initial evaluation of the efficacy of the 4 routes of administration was based on the flat field log values ​​of 6 different doses (25, 50, 100, 250, 500, 1000 μg/ml), and the route of administration (3.36) was superior to the therapeutic route (3.29). The therapeutic route was superior to the extra-corporal route (2.37), and the extra-vessel route was superior to the prophylactic route (0.72).
10. Other effects: Sodium alginate significantly promoted rat erythrocyte agglutination, with an agglutination rate of 53.63% and P<0.001 compared with the control group. Seaweed has a certain inhibitory effect on Bacillus subtilis.