New microscopy technology allows scientists to view cells through a silicon microfluidic device.
Scientists at the Massachusetts Institute of Technology (MIT) and the University of Texas at Arlington (UTA) have developed a new microscope that can view cell images from silicon, allowing them to accurately measure behind the wafer. Cell size and mechanical properties.
This new technology relies on near-infrared light, which helps scientists learn more about diseased or infected cells through silicon microfluidic devices.
Ishan Barman, one of the authors of the technical paper published by the former MIT Laser Biomedical Research Center (LBRC) and the October 2nd Science and Technology Report, said: "This technology is expected to incorporate cell visualization into silicon."
Other key authors of the paper include: former MIT postdoctoral fellow Narahara Chari Dingari, and UTA graduate student Bipin Joshi, Nelson Cardenas. Senior authors include: Umar Physics Assistant Professor Samarendra Mohanty. Other authors include: former MIT postdoctoral fellow, Jaqueline Soares, assistant professor at the Federal University of Ouro Preto, Brazil, and Ramachandra Rao Dasari, deputy director of LBRC.
Silicon is commonly used to build "on-chip lab" microfluidic devices that can be used for cell sorting and analysis based on their molecular properties and microelectronic devices. Barman, who is currently an assistant professor of mechanical engineering at Johns Hopkins University in the United States, said: "These devices have many potential applications in research and diagnostics, but if scientists can observe cell images in these devices, they may More useful."
To achieve this goal, Barman and his colleagues took advantage of the fact that silicon is transparent to infrared and near-infrared wavelengths of light. They adapted a microscopic technique called quantitative phase imaging that works by sending a laser beam through a sample that splits into two beams. By recombining the two beams and comparing the information carried by each beam, the researchers were able to determine the height of the sample and its refractive index. This is a measure of how light bends light as it passes through the material.
Traditional quantitative phase imaging uses a HeNe laser that produces visible light, but for the new system, the researchers used a titanium sapphire laser that tuned the infrared and near-infrared wavelengths. In this study, the researchers found that light with a wavelength of 980 nm is best used.
The researchers used the system to measure red blood cell height changes with nanometer-scale sensitivity through silicon wafers similar to those used in most electronic laboratories.
When red blood cells flow throughout the body, they tend to squeeze through very narrow blood vessels. When these cells are infected with malaria, they lose this deformability and form a blockage in tiny blood vessels. Dingari said the new microscopy technology can help scientists study how this happens. It can also be used to study the dynamics of malformed blood cells that cause sickle cell anemia.
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