Instrument Name: Gas Chromatography Mass Spectrometer
Instrument model: Agilent GC6890-5975I MS
Manufacturer: Agilent, USA
Method of use: gas phase with headspace sampler, FID detector;
The mass spectrum is the zui new quadrupole mass analyzer.
Specific use method:
First, the order of opening and closing:
Start up: nitrogen gas → power on → set temperature (column, vaporization) → heating → air, hydrogen → ignition → alignment baseline → injection
Shutdown: turn off hydrogen, air → turn off the heater → pass nitrogen to cool to room temperature → turn off the power → turn off the nitrogen
Second, the temperature setting
1, column temperature setting (range: -99 ° C ~ 399 ° C)
For example: set the temperature to 50 ° C, the command is as follows COL / AUX.1 I. TEMP 50 ENT
Injector temperature setting (range: 0 to 99 ° C)
For example: set the temperature to 120 ° C, the command is as follows INJ / AUX.2 120 ENT
2, understand the temperature setting of the column temperature:
COL/AUX.1 I.TEMP ENT
Sampler:
INJ/AUX.2 ENT
3. Monitor the actual temperature oven:
MONI COL/AUX.1
Sampler:
MONI INJ/AUX.2
4. After setting the temperature to start and stop the oven temperature and the vaporization chamber temperature, press the START button to start the temperature rise (to perform the program temperature, then press the START button).
To set the temperature control to automatically stop, the command is as follows:
For example, set STOPTIME to 5 hours (unit: minute)
SHIFT.D 2/STOP.T 300 ENT
Third, the detector
1 Select the detector and press DET 1 ENT in turn to select detector 1. If you know the number of the active detector, press DET ENT.
When understanding the detector type corresponding to the number, the command is:
MONI DET 1 ENT
2 Set the detector range:
Such as setting range 1: RANG 1 ENT
Show current usage range: RANG ENT
Fourth, analysis
When the column temperature and vaporization chamber temperature are set, press the START button to start heating up to the set temperature. When the column chamber temperature reaches within ±1 °C of the set temperature, the READY light is on. However, before the temperature reaches the set value, it will fluctuate slightly. The REDAY light will flash one or two times, but it will stabilize soon. When the temperature of the oven and vaporization chamber reaches the set temperature, the sample can be analyzed.
Fifth, data processing
1 Turning on the power switch All indicators light up for about 1 second, after which the indicator light flashes for about 30 seconds. In the meantime, if there is no bad condition, the READY indicator lights up.
2 Set the sensitivity of the recorder. If the sensitivity is set to 8: ATTEN 3 ENTER (see page 31 of the manual for comparison). Set the paper feed speed 10:
SPEED 10 ENTER.
3 Mark the chromatographic baseline operation SHIFT DOWN PLOT ENTER until the baseline of the chromatograph is stable. Once the SHIFT DOWN PLOT ENTER button is operated again, the drawing stops.
4 Print the deviation of the zero point of the chromatograph (unit: uV) Press the PRINT button, then press the CTRL button, and press the LEVEL button. Release the LEVEL button and release the CTRL button. Then press the ENTER key (note: the CTRL key must be pressed before the destination key LEVEL, and released after the destination key).
5 Adjust the zero point of the chromatograph to a range between -1000uV and +5000uV. Repeat 4 to 5 operations.
6 Move the pen to the home position and operate the ZERO ENTER button.
7 Injection Analysis Inject the sample into the chromatograph while pressing the START button.
Halloween Gummies,Halloween Gummy Candy,Halloween Gummy Worms,Jelly Halloween Gummy
Montreal Shantou Food Co., Ltd , https://www.montrealsnack.com