Chinese scholars reveal a new pattern of long-chain non-coding RNA cis-regulated gene expression
March 28, 2016 Source: Bio Valley
Window._bd_share_config={ "common":{ "bdSnsKey":{ },"bdText":"","bdMini":"2","bdMiniList":false,"bdPic":"","bdStyle":" 0","bdSize":"16"},"share":{ }};with(document)0[(getElementsByTagName('head')[0]||body).appendChild(createElement('script')) .src='http://bdimg.share.baidu.com/static/api/js/share.js?v=89860593.js?cdnversion='+~(-new Date()/36e5)];The international academic journal Cell Stem Cell recently published the latest research results of the research group "Divergent lnc RNAs regulate gene expression and lineage differentiation in pluripotent cells" of Tsinghua University Medical College. RNA regulates gene expression and pluripotent stem cell differentiation. The system reveals the expression of adjacent genes in the cis-regulated genome of long-chain non-coding RNAs and their role in stem cell differentiation and development. The research work was funded by the National Natural Science Foundation of China (31471219, 8141101062). The paper link: http://(16)00062-X.
Extensive transcription of higher eukaryotic genomes produces a large number of long-chain non-coding RNAs (lncRNAs) that do not have the function of encoding proteins. At present, people's understanding of them is still very limited, and lncRNA is considered to be a dark substance in biology. The functional classification and prediction of LncRNA is an important issue that has been pursued and explored in the field of non-coding RNA. It plays an important role in understanding the biological function and existence of non-coding RNA.
Shen Xiaoyu's team found that the distribution of lncRNAs on the genome was not random and was classified according to their positional relationship with the genes encoded by adjacent proteins on the genome. Among them, antisense long-chain non-coding RNAs (divergentlncRNAs) and adjacent protein genes are reversely arranged and transcribed in a head-to-head manner on the genome. They account for 20% of the total number of human and murine lncRNAs, and are more likely to be distributed in the vicinity of coding transcription factors and developmental regulatory genes, which are evolutionarily older than lncRNAs far from protein-encoding genes. Surprisingly, silencing 75% of randomly selected antisense long-chain non-coding RNAs resulted in decreased expression of adjacent protein genes. The group used lncRNAEvx1as as an example to further analyze the molecular mechanism of antisense long-chain non-coding RNA. Evx1asRNA binds in situ to the DNA region of its own transcription, recruits the transcriptional activation cofactor Mediator, promotes an activated chromatin epigenetic modification and the formation of high-level structures, providing a “window of opportunity†for the rapid activation of EVX1 to integrate various Signals, thereby positively regulating the transcription of EVX1 genes. Interference with the expression of lncRNAEvx1as severely impedes the differentiation of pluripotent stem cells. Therefore, antisense long-chain non-coding RNA, at least a considerable part of it, can cis-regulate the transcription of genes encoding adjacent proteins, precisely control the spatiotemporal expression of these developmental gene loci, and participate in the development and related Other biological processes.
This study found that from a higher level, it is revealed that lcisRNAs cis-regulated adjacent protein-encoding genes are a new mode of transcriptional regulation. Based on the above cis-regulation rules, one can predict the function of a large number of unidentified non-coding lncRNAs based on the function of genes encoding nearby known proteins. This functional prediction will help researchers better design experiments and study unknown lncRNAs, which is important for a comprehensive understanding of non-coding genome functions, gene expression regulation and organism development.
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The automatic enzyme immunoassay analyzer is based on the principle that the enzyme and the substrate can produce a color reaction, the absorption lines of different substances have different characteristics, and strictly abide by the Lambert-Beer law, quantitative and qualitative analysis of substances. instrument. The method of analyzing the content of various enzymes such as antigen or antibody generally mainly adopts colorimetric method. In practice, spectrophotometry is the basic working principle of an automatic enzyme immunoassay analyzer. The light emitted by the light source lamp becomes a beam of monochromatic light after passing through a filter or a monochromator. The monochromatic light beam passes through the sample to be tested in the microtiter plate, and part of the monochromatic light beam is absorbed by the sample and reaches the photodetector. The intensity of the light signal projected on it is converted into the magnitude of the electrical signal by the photodetector. This electrical signal is processed by pre-amplification, logarithmic amplification, analog-to-digital conversion, etc., and then sent to the microprocessor for data processing and calculation, and the test results are output by the display and printer. The microprocessor completes the movement in the X and Y directions of the mechanical drive through the control circuit.
The automatic enzyme immunoassay analyzer adds the sample to the microwells of the pre-coated antigen or antibody microtiter plate, washes after the reaction, removes the unseparated ligand, then adds the enzyme isolate, after incubation, washes again , remove the unseparated compound, and then add the enzyme substrate, after the reaction, the colored final product is formed, and the stop solution is added to stop the reaction. The absorbance of each microwell of the microtiter plate is read by the wavelength that has been set by the spectrophotometer. The concentration value of the analyte in the sample is calculated by the absorbance value of the sample and the standard curve, so that the quantitative result can be obtained, or the absorbance of the sample is compared with that of the standard product, so that the positive or negative qualitative result can be obtained.
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