In vivo electroporation is the introduction of a foreign gene into an animal's target tissue or organ by an electric field. This method can effectively introduce foreign genes, can be applied to a variety of tissues and organs, and is highly efficient. The principle of the living electroporation method is very simple. At the moment of the DC electric field, the surface of the cell membrane produces a hydrophobic or hydrophilic microchannel of 105 to 115 μm. This channel can be maintained for several milliseconds to several seconds and then recovered by itself. During this time, macromolecules such as DNA can enter the cell through this tiny channel. In recent years, the number of reports on transgenic research by living electroporation has been increasing, and the advantages in gene therapy are becoming more and more obvious. It is a good method for introducing living genes.
In the gene therapy, the use of in vivo electroporation to introduce antibodies or chemotherapeutic drugs into tumors, promote the delivery of antibodies and chemotherapeutic drugs for treatment, and now replace the application of antibodies with specific foreign genes, simplifying the operation steps and reducing the number of steps. cost.
First, in the treatment of skin tumors and brain tumors, electroporation can effectively introduce a drug gene into a treatment site. After electroporation introduced human monocyte chemoattractant protein gene into rat brain tumor, the protein was expressed locally in the tumor, and aggregation of macrophages and leukocytes was observed at the site of gene introduction.
At the same time, scientists have studied the role of electroporation in the treatment of tumors from another angle. The steep pulse of high electric field intensity is used to induce irreversible electrical breakdown of tumor cells and eventually die. This method is very suitable for the treatment of local tissue tumors.
In addition, the living electroporation method can also participate in the treatment of cancer. After the gene is introduced into the body by the living electroporation method, the introduced tissue can become a temporary secretory organ that secretes antibodies and the like to resist the erosion or compensation of cancer cells. The loss of various physiological functions of the body caused by erosion.
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