Keywords: Endra Nexus 128, photoacoustic imaging, nanoprobe, gold nanorod, tumor
Overview: The Photoacoustic Imaging System (Endra Nexus 128) features non-invasive detection and is also ideal for continuous observation of experimental animals because it is true 3-D imaging. After the gold nanorods are injected into the experimental animals, the experimental animals can be scanned intermittently, thereby obtaining dynamic information that the probes are ingested, absorbed, and removed in the tumor.
Objective: To study the dynamic distribution of gold nanorods in transplanted tumors in mice.
Experimental material: The photoacoustic imaging system was an Endra Nexus 128 system (Endra Inc., USA). The nanoprobe uses self-made gold nanorods and has a strong absorption peak at 680 nm. Mice implanted with U87Luc2 Glioma.
Experimental methods and results:
1 Turn on the Endra Nexus 128 Photoacoustic Imaging System and enter the normal photoacoustic scanning experiment after half an hour of warm-up.
2 The mice were placed in an anesthesia box, and 100 ul of gold nanorods were injected through the tail vein after anesthesia.
3 Place the mouse on the animal tray so that the tumor site is toward the groove in the center of the tray. Add about 900 ul of water to the mouse with a pipette to couple the tumor to water to facilitate the transmission of ultrasound.
4 Place the tray in the scanning room of the photoacoustic imaging system, place the tray on the high (subcutaneous tumor) card slot, and fix it by rotation. Connect the gas anesthesia machine tubing to the snout of the mouse.
5 Close the scan room door.
6 Set the scanning parameters on the PC, scan every 30 degrees for 3 seconds, for a total of about 36 seconds. Animals were scanned every 15 minutes for a total of 5 scans for a total of 75 minutes. Image reconstruction is then performed on the PC and the data is pushed to the MAC machine that analyzes the data.
7 Using the Osirix software on a Mac, select the tumor area and select the tumor volume. Using the probe uptake software developed by Endra itself, the intensity of the photoacoustic signal is analyzed and the corresponding dynamic curve is generated.
8 Use the 3D reconstruction function of OSIRIX to perform 3D rendering of tumors.
Experimental results: It can be seen from the interface of software analysis that Nexus 128 can realize all aspects of probe experimental research, including comparing the absorption time of different probes, comparing the absorption time of different regions of the tumor, and testing the concentration of probes at different absorption times. And to check the 3-D distribution of the targeted probe inside the tumor, and the like. These applications are critical for probe development and preclinical evaluation.
YT-H711
YT-H711
Shenzhen Sunshine Technology Co.,Ltd , https://www.szyatwin.com