The liquid paraffin preservation method uses chemical liquid paraffin, which is put into a 40° C. incubator for several hours or in a desiccator for several days after autoclaving, and the liquid paraffin is aspirated with a sterile pipette or syringe and injected into the strain test tube. The injection volume should be about 1 cm higher than the tip of the bevel, stuffed with a tampon, sealed with wax, stored upright at a low temperature, and kept for 5 years.
The bran preservation method weighs a certain amount of bran and mixes with water or nutrient solution before use. The water and bran are put into test tubes, and then inserted into the strains after autoclaving. The cultures are placed at a suitable temperature. After the mycelium has developed, the test tube is dried at room temperature. Then place it in a dry place below 20°C and keep it for 3-5 years.
Physiological saline storage method to connect the strains into the culture medium of the potato, 60 ml of culture liquid per 250 ml conical flask, shaking culture or shaking 5-10 times a day, culture for 5-7 days, then the formed mycelium ball is moved into In a test tube of 5 ml of sterile physiological saline, about 4-5 pieces of mycelial balls were transferred into each tube, and the tampon was plugged, and the tube was sealed with a wax and stored under low temperature for 1 year.
The wheat preservation method takes high-quality wheat, soaked in water at 20°C for 5 hours after washing, a little air-drying, and the tube is loaded, and the loading amount is 1/4 to 1/3 of the tube length, and sterilization is suitable. Scatter, after cooling enter the mycelium, suitable temperature culture until sparse hyphae appear on the majority of wheat grain termination. Store in a dry place below 25°C for 1-2 years.
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