Pipette pipetting techniques for laboratory instruments 6 – water infusion time and pipetting rate

I believe that every laboratory worker has experience in using pipettes , and also requires high accuracy and accuracy of the pipette, but in addition to the accuracy and accuracy of the pipette itself will affect our shift. In addition to the liquid results, the use of pipettes can also affect our pipetting results. In order to get the best results and ensure the accuracy of the experiment, we need Use good pipetting techniques. Lebe will introduce you to tips and tricks for getting the best pipetting results.
Tip 1 : Tip into the water time
The time of water in the sample is a key factor in good pipetting techniques when the sample is drawn into the tip. After the locating pin is released smoothly, a short pause must be made in the sample before the pipette tip is removed from the sample to ensure that the full sample is drawn into the tip. If the amount is large and for viscous substances, it must be taken out after waiting for a long time, because the gas chamber in the pipette takes one second to achieve equilibrium.


Tip 2 : Aspiration rate
The pipetting rates of different pipettes are not the same, so it will affect the accuracy and accuracy of the pipetting results. Our user should release the pipette locating pin button in a smooth and controlled manner. If the locating pin is released too quickly, the consequence may be that the aerosol will enter the shank of the pipette and damage the internal components of the pipette. This phenomenon is particularly noticeable when liquid splatters are present. If the liquid is corrosive, it may damage the handle of the pipette and cause cross-contamination of the pipetting sample.

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