Liuwei Dihuang Capsule is a compound preparation consisting of 6 Chinese herbal medicines of Rehmannia glutinosa, Hawthorn, Mudan, Yam, Sui and Alisma. It has the effect of nourishing yin and tonifying kidney. It is used to treat kidney yin loss, dizziness, tinnitus, waist and knees, bone. Steaming hot flashes, night sweats and nocturnal emission embolism. The determination of the content of ursolic acid in the main drug yam is one of the main means to control the quality of the drug. The Chinese Pharmacopoeia is measured by thin-layer scanning method, which is cumbersome, complicated, time-consuming and laborious, accurate and heavy. Poor in current. The author uses high performance liquid chromatography to determine its content. The method has the characteristics of simple operation, rapidity, accuracy and reproducibility.
Testing instruments: high performance liquid chromatography, including solvent infusion pumps, UV-visible detectors, manual injectors, chromatography data stations.
Chromatographic conditions: column temperature: room temperature; mobile phase: methanol-water-formic acid (90:10:0.02); flow rate: 1.0 mL/min; detection wavelength: 220 nm; injection amount: 10 μL.
Solution preparation
Reference solution: accurately weigh 10mg of ursolic acid reference substance, put it in a 10mL volumetric flask, add appropriate amount of methanol, dissolve, dilute to the mark with methanol, shake well, as a reference solution (about 1mg of ursolic acid per 1mL of methanol) ).
Test solution: accurately weigh 2g of this product, put it in the Soxhlet extractor, heat and reflux until colorless, about 5h, the extract is recovered to dry, the residue is dissolved with appropriate amount of methanol, and transferred to a 10nL volumetric flask with methanol. Dilute to the mark with methanol and centrifuge (1200r/min) for 10min. Take the supernatant as the test solution.
Negative control solution: Weigh out other medicinal materials other than hawthorn according to the prescription ratio, and obtain a negative sample according to the production method, and prepare a negative control solution according to the preparation method of the test solution.
The sample solution was measured under the operating conditions of the above apparatus.
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